stem-cells-training-researchers-human-iPSCs

The Center provides users with a clean, dedicated space specifically for culturing human induced pluripotent stem cells (iPSCs), occupying approximately 1,250 square feet of space on the second floor of Porter Biosciences, on the CU Boulder main campus.Ìý

We provide:

  • 5ÌýBiosafety Cabinets
  • 7 CO2Ìýand tri-gasÌýincubators
  • Automated Fluorescence Cell Counter
  • Inverted Fluorescence Scanner Microscope (Olympus IX83)Ìýwith live-cell time-lapse capabilitiesÌý(Live and fixed cell imaging in monolayer or suspensions, organoids, embryos, organotypic slides, and more!)
  • Sterile Dissection Hood equipped with a dissecting Olympus CKX53 microscope and heated/CO2Ìýstage for work with live cultures and live/fix imagine
  • OlympusÌýProviÌýCM20Ìý(imaging and monitoring cell confluence, cell count, and cell culture data records)
  • EVOS7000 Microscope
  • CO2 shaker + Remote Controller for Organoids Derivation
  • Transfection Systems (Neon NxT Electroporation &ÌýAmaxaÌýNucleofector)
  • Phase Contrast MicroscopesÌý
  • Liquid Nitrogen Cryostorage Space
  • 6 Water/Beads Bath andÌýThawSTARÌýAutomated Cell Thawing System
  • Centrifuges and Microcentrifuges
  • Plate shakers
  • Block heathers (microcentrifuge tubes)
  • Mr. FrostyÌý&ÌýCoolCellÌýÌýLX Cell Freezing Containers
  • Bioengineering SpaceÌý
  • Standard iPSC consumables (i.e., pipettes, plasticware, media, substrates, etc.) are readily available to users.Ìý
  • Reagents for sale (even 30% less)Ìý
  • Training of new users
  • hiPSC training. Ìý ***Coming soon: Neural Culture workshop!
  • Microscope training
  • Refrigerators &Ìýfreezers (4OC, -20oC, -80oC)

Stem cell center user using a microscope to inspect cells.

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